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human foreskin fibroblast cell line hff 1  (ATCC)
99
ATCC human foreskin fibroblast cell line hff 1
(A) Metabolic activity of <t>the</t> <t>HFF-1</t> cells was assessed after 16 h of treatment with increasing concentrations of the Hsp70 inhibitors or the equivalent volume of the DMSO control. The percentage of metabolically active cells is presented relative to the DMSO control. A decrease of 15% in metabolically active cells was considered non-toxic (dotted line represents 85%). (B) HFF-1 cells were infected with CHIKV-LR OPY1 at MOI 5 while treated with 2 µM or 1 µM of JG98 or JG345, respectively, and virus production was assessed at 9 hpi via plaque assay. Data are presented as mean±SEM from three independent experiments, and statistical differences were determined via One-way ANOVA and presented when p≤0.05 with *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.001.
Human Foreskin Fibroblast Cell Line Hff 1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human foreskin fibroblast cell line hff 1/product/ATCC
Average 99 stars, based on 1 article reviews
human foreskin fibroblast cell line hff 1 - by Bioz Stars, 2026-02
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t gondii strains human foreskin fibroblast cells  (ATCC)
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ATCC t gondii strains human foreskin fibroblast cells
(A) Metabolic activity of <t>the</t> <t>HFF-1</t> cells was assessed after 16 h of treatment with increasing concentrations of the Hsp70 inhibitors or the equivalent volume of the DMSO control. The percentage of metabolically active cells is presented relative to the DMSO control. A decrease of 15% in metabolically active cells was considered non-toxic (dotted line represents 85%). (B) HFF-1 cells were infected with CHIKV-LR OPY1 at MOI 5 while treated with 2 µM or 1 µM of JG98 or JG345, respectively, and virus production was assessed at 9 hpi via plaque assay. Data are presented as mean±SEM from three independent experiments, and statistical differences were determined via One-way ANOVA and presented when p≤0.05 with *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.001.
T Gondii Strains Human Foreskin Fibroblast Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t gondii strains human foreskin fibroblast cells/product/ATCC
Average 99 stars, based on 1 article reviews
t gondii strains human foreskin fibroblast cells - by Bioz Stars, 2026-02
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human foreskin fibroblast cells  (ATCC)
99
ATCC human foreskin fibroblast cells
(A) Metabolic activity of <t>the</t> <t>HFF-1</t> cells was assessed after 16 h of treatment with increasing concentrations of the Hsp70 inhibitors or the equivalent volume of the DMSO control. The percentage of metabolically active cells is presented relative to the DMSO control. A decrease of 15% in metabolically active cells was considered non-toxic (dotted line represents 85%). (B) HFF-1 cells were infected with CHIKV-LR OPY1 at MOI 5 while treated with 2 µM or 1 µM of JG98 or JG345, respectively, and virus production was assessed at 9 hpi via plaque assay. Data are presented as mean±SEM from three independent experiments, and statistical differences were determined via One-way ANOVA and presented when p≤0.05 with *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.001.
Human Foreskin Fibroblast Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human foreskin fibroblast cells/product/ATCC
Average 99 stars, based on 1 article reviews
human foreskin fibroblast cells - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier
normal human foreskin fibroblast hff 1 cells  (ATCC)
99
ATCC normal human foreskin fibroblast hff 1 cells
(A) Metabolic activity of <t>the</t> <t>HFF-1</t> cells was assessed after 16 h of treatment with increasing concentrations of the Hsp70 inhibitors or the equivalent volume of the DMSO control. The percentage of metabolically active cells is presented relative to the DMSO control. A decrease of 15% in metabolically active cells was considered non-toxic (dotted line represents 85%). (B) HFF-1 cells were infected with CHIKV-LR OPY1 at MOI 5 while treated with 2 µM or 1 µM of JG98 or JG345, respectively, and virus production was assessed at 9 hpi via plaque assay. Data are presented as mean±SEM from three independent experiments, and statistical differences were determined via One-way ANOVA and presented when p≤0.05 with *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.001.
Normal Human Foreskin Fibroblast Hff 1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/normal human foreskin fibroblast hff 1 cells/product/ATCC
Average 99 stars, based on 1 article reviews
normal human foreskin fibroblast hff 1 cells - by Bioz Stars, 2026-02
99/100 stars
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human foreskin fibroblast cell line  (ATCC)
99
ATCC human foreskin fibroblast cell line
(A) Metabolic activity of <t>the</t> <t>HFF-1</t> cells was assessed after 16 h of treatment with increasing concentrations of the Hsp70 inhibitors or the equivalent volume of the DMSO control. The percentage of metabolically active cells is presented relative to the DMSO control. A decrease of 15% in metabolically active cells was considered non-toxic (dotted line represents 85%). (B) HFF-1 cells were infected with CHIKV-LR OPY1 at MOI 5 while treated with 2 µM or 1 µM of JG98 or JG345, respectively, and virus production was assessed at 9 hpi via plaque assay. Data are presented as mean±SEM from three independent experiments, and statistical differences were determined via One-way ANOVA and presented when p≤0.05 with *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.001.
Human Foreskin Fibroblast Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human foreskin fibroblast cell line/product/ATCC
Average 99 stars, based on 1 article reviews
human foreskin fibroblast cell line - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier
human foreskin fibroblast hff 1 cells  (ATCC)
99
ATCC human foreskin fibroblast hff 1 cells
(A) Metabolic activity of <t>the</t> <t>HFF-1</t> cells was assessed after 16 h of treatment with increasing concentrations of the Hsp70 inhibitors or the equivalent volume of the DMSO control. The percentage of metabolically active cells is presented relative to the DMSO control. A decrease of 15% in metabolically active cells was considered non-toxic (dotted line represents 85%). (B) HFF-1 cells were infected with CHIKV-LR OPY1 at MOI 5 while treated with 2 µM or 1 µM of JG98 or JG345, respectively, and virus production was assessed at 9 hpi via plaque assay. Data are presented as mean±SEM from three independent experiments, and statistical differences were determined via One-way ANOVA and presented when p≤0.05 with *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.001.
Human Foreskin Fibroblast Hff 1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human foreskin fibroblast hff 1 cells/product/ATCC
Average 99 stars, based on 1 article reviews
human foreskin fibroblast hff 1 cells - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier
human foreskin fibroblast hff cell line  (ATCC)
99
ATCC human foreskin fibroblast hff cell line
(A) Metabolic activity of <t>the</t> <t>HFF-1</t> cells was assessed after 16 h of treatment with increasing concentrations of the Hsp70 inhibitors or the equivalent volume of the DMSO control. The percentage of metabolically active cells is presented relative to the DMSO control. A decrease of 15% in metabolically active cells was considered non-toxic (dotted line represents 85%). (B) HFF-1 cells were infected with CHIKV-LR OPY1 at MOI 5 while treated with 2 µM or 1 µM of JG98 or JG345, respectively, and virus production was assessed at 9 hpi via plaque assay. Data are presented as mean±SEM from three independent experiments, and statistical differences were determined via One-way ANOVA and presented when p≤0.05 with *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.001.
Human Foreskin Fibroblast Hff Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human foreskin fibroblast hff cell line/product/ATCC
Average 99 stars, based on 1 article reviews
human foreskin fibroblast hff cell line - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier
human foreskin fibroblast hff cells  (ATCC)
99
ATCC human foreskin fibroblast hff cells
(A) Metabolic activity of <t>the</t> <t>HFF-1</t> cells was assessed after 16 h of treatment with increasing concentrations of the Hsp70 inhibitors or the equivalent volume of the DMSO control. The percentage of metabolically active cells is presented relative to the DMSO control. A decrease of 15% in metabolically active cells was considered non-toxic (dotted line represents 85%). (B) HFF-1 cells were infected with CHIKV-LR OPY1 at MOI 5 while treated with 2 µM or 1 µM of JG98 or JG345, respectively, and virus production was assessed at 9 hpi via plaque assay. Data are presented as mean±SEM from three independent experiments, and statistical differences were determined via One-way ANOVA and presented when p≤0.05 with *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.001.
Human Foreskin Fibroblast Hff Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human foreskin fibroblast hff cells/product/ATCC
Average 99 stars, based on 1 article reviews
human foreskin fibroblast hff cells - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier

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(A) Metabolic activity of the HFF-1 cells was assessed after 16 h of treatment with increasing concentrations of the Hsp70 inhibitors or the equivalent volume of the DMSO control. The percentage of metabolically active cells is presented relative to the DMSO control. A decrease of 15% in metabolically active cells was considered non-toxic (dotted line represents 85%). (B) HFF-1 cells were infected with CHIKV-LR OPY1 at MOI 5 while treated with 2 µM or 1 µM of JG98 or JG345, respectively, and virus production was assessed at 9 hpi via plaque assay. Data are presented as mean±SEM from three independent experiments, and statistical differences were determined via One-way ANOVA and presented when p≤0.05 with *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.001.

Journal: bioRxiv

Article Title: The Heat shock protein 70 machinery is crucial in the production of infectious chikungunya virus progeny

doi: 10.64898/2026.01.26.701664

Figure Lengend Snippet: (A) Metabolic activity of the HFF-1 cells was assessed after 16 h of treatment with increasing concentrations of the Hsp70 inhibitors or the equivalent volume of the DMSO control. The percentage of metabolically active cells is presented relative to the DMSO control. A decrease of 15% in metabolically active cells was considered non-toxic (dotted line represents 85%). (B) HFF-1 cells were infected with CHIKV-LR OPY1 at MOI 5 while treated with 2 µM or 1 µM of JG98 or JG345, respectively, and virus production was assessed at 9 hpi via plaque assay. Data are presented as mean±SEM from three independent experiments, and statistical differences were determined via One-way ANOVA and presented when p≤0.05 with *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.001.

Article Snippet: The human bone osteosarcoma epithelial cell line U2OS (ATCC HTB-96) and human foreskin fibroblast cell line HFF-1 (ATCC SCRC-1041) were maintained in Dulbecco’s minimal essential medium (DMEM), high glucose, GlutaMAX tm, and sodium pyruvate (Gibco), supplemented with penicillin-streptomycin (P/S; 100U/mL, Gibco) and 10% or 15% fetal bovine serum (FBS, Lonza), respectively.

Techniques: Activity Assay, Control, Metabolic Labelling, Infection, Virus, Plaque Assay

Metabolic activity of (A) U2OS and (B) HFF-1 cells was assessed after 16 h of treatment with increasing concentrations of JG231 or the equivalent volume of the DMSO control. The percentage of metabolically active cells is presented relative to the DMSO control. A decrease of 15% in metabolically active cells was considered non-toxic (dotted line represents 85%). (C) U2OS cells and (D) HFF-1 cells were infected with CHIKV-LR for 9 h in the presence of 2 µM JG231 or the DMSO control. Supernatants were collected, and the number of infectious particles in the supernatant was determined using the plaque assay. (E and F) Mouse skin explants were infected with 10 6 PFU/mL of the CHIKV 899 strain during treatment with 2 µM JG231 or an equal volume of DMSO. (E) Infectious virus production (tissue culture infectious dose 50% (TCID 50 )) and (F) total virus production (GEC) at 2 dpi is displayed per mg tissue. The dotted line indicates the limit of quantification (LOQ). To evaluate statistical differences from the DMSO control, we used a Student T-test, and differences are presented when p≤0.05 with *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.001.

Journal: bioRxiv

Article Title: The Heat shock protein 70 machinery is crucial in the production of infectious chikungunya virus progeny

doi: 10.64898/2026.01.26.701664

Figure Lengend Snippet: Metabolic activity of (A) U2OS and (B) HFF-1 cells was assessed after 16 h of treatment with increasing concentrations of JG231 or the equivalent volume of the DMSO control. The percentage of metabolically active cells is presented relative to the DMSO control. A decrease of 15% in metabolically active cells was considered non-toxic (dotted line represents 85%). (C) U2OS cells and (D) HFF-1 cells were infected with CHIKV-LR for 9 h in the presence of 2 µM JG231 or the DMSO control. Supernatants were collected, and the number of infectious particles in the supernatant was determined using the plaque assay. (E and F) Mouse skin explants were infected with 10 6 PFU/mL of the CHIKV 899 strain during treatment with 2 µM JG231 or an equal volume of DMSO. (E) Infectious virus production (tissue culture infectious dose 50% (TCID 50 )) and (F) total virus production (GEC) at 2 dpi is displayed per mg tissue. The dotted line indicates the limit of quantification (LOQ). To evaluate statistical differences from the DMSO control, we used a Student T-test, and differences are presented when p≤0.05 with *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.001.

Article Snippet: The human bone osteosarcoma epithelial cell line U2OS (ATCC HTB-96) and human foreskin fibroblast cell line HFF-1 (ATCC SCRC-1041) were maintained in Dulbecco’s minimal essential medium (DMEM), high glucose, GlutaMAX tm, and sodium pyruvate (Gibco), supplemented with penicillin-streptomycin (P/S; 100U/mL, Gibco) and 10% or 15% fetal bovine serum (FBS, Lonza), respectively.

Techniques: Activity Assay, Control, Metabolic Labelling, Infection, Plaque Assay, Virus